› 3fho

X-ray diffraction
2.8Å resolution

Structure of S. pombe Dbp5

Released:
DOI: 10.2210/pdb3fho/pdb
PDB entry 3fho coloured by chain, front view.
PDB entry 3fho coloured by chain, side view.
PDB entry 3fho coloured by chain, top view.
Source organism: Schizosaccharomyces pombe
Primary publication:
Solution and crystal structures of mRNA exporter Dbp5p and its interaction with nucleotides.
Fan JS, Cheng Z, Zhang J, Noble C, Zhou Z, Song H, Yang D
J Mol Biol 388 1-10 (2009)
PMID: 19281819

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170818 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent RNA helicase dbp5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 508 amino acids
Theoretical weight: 53.09 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q09747 (Residues: 139-503; Coverage: 73%)
Gene names: SPBC12C2.06, dbp5
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 108.736Å b: 144.044Å c: 79.137Å
α: 90° β: 89.96° γ: 90°
R-values:
R R work R free
0.292 0.29 0.327
Expression system: Escherichia coli BL21

Quick links

Citations

12 review citations

From unwinding to clamping - the DEAD box RNA helicase family.
Linder et al. (2011)
11 more

2 mentions without citation

A conserved mechanism of DEAD-box ATPase activation by nucleoporins and InsP6 in mRNA export.
Montpetit et al. (2011)
1 more