PDBe 3fga

X-ray diffraction
2.7Å resolution

Structural Basis of PP2A and Sgo interaction

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform Chain: A
Molecule details ›
Chain: A
Length: 588 amino acids
Theoretical weight: 65.26 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q76MZ3 (Residues: 2-589; Coverage: 100%)
Gene name: Ppp2r1a
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform Chain: B
Molecule details ›
Chain: B
Length: 403 amino acids
Theoretical weight: 47.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13362 (Residues: 34-436; Coverage: 77%)
Gene names: KIAA0044, PPP2R5C
Structure domains: Leucine-rich Repeat Variant
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform Chain: C
Molecule details ›
Chain: C
Length: 309 amino acids
Theoretical weight: 35.64 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P67775 (Residues: 1-309; Coverage: 100%)
Gene name: PPP2CA
Sequence domains: Calcineurin-like phosphoesterase
Structure domains: Purple Acid Phosphatase; chain A, domain 2
Shugoshin 1 Chain: D
Molecule details ›
Chain: D
Length: 47 amino acids
Theoretical weight: 5.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5FBB7 (Residues: 51-96; Coverage: 8%)
Gene names: SGO1, SGOL1
Structure domains: Single helix bin
MICROCYSTIN-LR Chain: E
Molecule details ›
Chain: E
Length: 7 amino acids
Theoretical weight: 1.01 KDa
Source organism: Microcystis aeruginosa
Expression system: Not provided

Ligands and Environments

1 bound ligand:

3 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C2221
Unit cell:
a: 104.94Å b: 145.856Å c: 294.153Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.228 0.277
Expression systems:
  • Escherichia coli
  • Trichoplusia ni
  • Not provided