PDBe 3ffc

X-ray diffraction
2.8Å resolution

Crystal Structure of CF34 TCR in complex with HLA-B8/FLR

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, B-8 alpha chain Chains: A, F
Molecule details ›
Chains: A, F
Length: 277 amino acids
Theoretical weight: 32.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P30460 (Residues: 25-301; Coverage: 82%)
Gene names: HLA-B, HLAB
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, G
Molecule details ›
Chains: B, G
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
FLRGRAYGL peptide from an EBV protein Chains: C, H
Molecule details ›
Chains: C, H
Length: 9 amino acids
Theoretical weight: 1.05 KDa
CF34 alpha chain Chains: D, I
Molecule details ›
Chains: D, I
Length: 202 amino acids
Theoretical weight: 22.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
T cell receptor beta constant 1 Chains: E, J
Molecule details ›
Chains: E, J
Length: 247 amino acids
Theoretical weight: 27.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01850 (Residues: 1-129; Coverage: 73%)
Gene name: TRBC1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: I222
Unit cell:
a: 111.564Å b: 171.807Å c: 272.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.221 0.268
Expression system: Escherichia coli