PDBe 3fey

X-ray diffraction
2.2Å resolution

Crystal structure of the CBC-importin alpha complex.

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Nuclear cap-binding protein subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 790 amino acids
Theoretical weight: 91.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q09161 (Residues: 1-790; Coverage: 100%)
Gene names: CBP80, NCBP, NCBP1
Sequence domains:
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
Nuclear cap-binding protein subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 156 amino acids
Theoretical weight: 18.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P52298 (Residues: 1-156; Coverage: 100%)
Gene names: CBP20, NCBP2, PIG55
Sequence domains: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Structure domains: Alpha-Beta Plaits
Importin subunit alpha-1 Chain: C
Molecule details ›
Chain: C
Length: 467 amino acids
Theoretical weight: 50.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P52292 (Residues: 70-529; Coverage: 87%)
Gene names: KPNA2, RCH1, SRP1
Sequence domains:
Structure domains: Leucine-rich Repeat Variant

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 83.842Å b: 104.505Å c: 108.558Å
α: 90° β: 109.04° γ: 90°
R-values:
R R work R free
0.194 0.192 0.238
Expression system: Escherichia coli