PDBe 3f8j

X-ray diffraction
1.99Å resolution

Mouse UHRF1 SRA domain bound with hemi-methylated CpG, crystal structure in space group C222(1)

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
E3 ubiquitin-protein ligase UHRF1 Chain: B
Molecule details ›
Chain: B
Length: 212 amino acids
Theoretical weight: 23.86 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8VDF2 (Residues: 417-628; Coverage: 27%)
Gene names: Np95, Uhrf1
Sequence domains: SAD/SRA domain
Structure domains: SRA-YDG
5'-D(*DCP*DCP*DAP*DTP*DGP*(5CM)P*DGP*DCP*DTP*DGP*DAP*DC)-3' Chain: F
Molecule details ›
Chain: F
Length: 12 nucleotides
Theoretical weight: 3.64 KDa
Source organism: Mus musculus
Expression system: Not provided
5'-D(*DGP*DTP*DCP*DAP*DGP*DCP*DGP*DCP*DAP*DTP*DGP*DG)-3' Chain: G
Molecule details ›
Chain: G
Length: 12 nucleotides
Theoretical weight: 3.7 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2221
Unit cell:
a: 61.67Å b: 91.59Å c: 118.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.232
Expression systems:
  • Escherichia coli
  • Not provided