PDBe 3epp

X-ray diffraction
2.41Å resolution

Crystal structure of mRNA cap guanine-N7 methyltransferase (RNMT) in complex with sinefungin

Released:
Source organism: Homo sapiens
Entry authors: Amaya MF, Zeng H, Loppnau P, Bountra C, Weigelt J, Arrowsmith CH, Edwards AM, Botchkarev A, Min J, Plotnikov AN, Wu H, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(7)G(5')pppR-RNA
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
mRNA cap guanine-N7 methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 313 amino acids
Theoretical weight: 36.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43148 (Residues: 165-476; Coverage: 66%)
Gene names: KIAA0398, RNMT
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SFG 2 x SFG
No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E DW
Spacegroup: P212121
Unit cell:
a: 58.515Å b: 73.65Å c: 147.518Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.228 0.3
Expression system: Escherichia coli