PDBe 3ej8

X-ray diffraction
2.55Å resolution

Structure of double mutant of human iNOS oxygenase domain with bound immidazole

Released:

Function and Biology Details

Reaction catalysed:
(1a) 2 L-arginine + 2 NADPH + 2 O(2) = 2 N(omega)-hydroxy-L-arginine + 2 NADP(+) + 2 H(2)O
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitric oxide synthase, inducible Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 424 amino acids
Theoretical weight: 49.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35228 (Residues: 82-505; Coverage: 37%)
Gene names: NOS2, NOS2A
Sequence domains: Nitric oxide synthase, oxygenase domain
Structure domains:

Ligands and Environments


Cofactor: Ligand HEC 4 x HEC
3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P212121
Unit cell:
a: 89.883Å b: 150.71Å c: 191.047Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.232 0.271
Expression system: Escherichia coli