› 3e4b

X-ray diffraction
2.5Å resolution

Crystal structure of AlgK from Pseudomonas fluorescens WCS374r

Released:
DOI: 10.2210/pdb3e4b/pdb
PDB entry 3e4b coloured by chain, front view.
PDB entry 3e4b coloured by chain, side view.
PDB entry 3e4b coloured by chain, top view.
Source organism: Pseudomonas fluorescens
Primary publication:
AlgK is a TPR-containing protein and the periplasmic component of a novel exopolysaccharide secretin.
Keiski CL, Harwich M, Jain S, Neculai AM, Yip P, Robinson H, Whitney JC, Riley L, Burrows LL, Ohman DE, Howell PL
Structure 18 265-73 (2010)
PMID: 20159471

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163503 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AlgK Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 452 amino acids
Theoretical weight: 49.62 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli
Structure domains: Tetratricopeptide repeat domain

Ligands and Environments

2 bound ligands:
Ligand CL 3 x CL
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 79.09Å b: 107.82Å c: 119.03Å
α: 90° β: 96.97° γ: 90°
R-values:
R R work R free
0.22 0.22 0.28
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Microbial alginate production, modification and its applications.
Hay et al. (2013)
8 more

8 mentions without citation

Synthase-dependent exopolysaccharide secretion in Gram-negative bacteria.
Whitney et al. (2013)
7 more