PDBe 3e3q

X-ray diffraction
2.95Å resolution

Structure of the 3alpham13 high-affinity mutant of the 2C TCR in complex with Ld/QL9

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
QL9 peptide Chains: G, K, O, Q, T, X, b, f
Molecule details ›
Chains: G, K, O, Q, T, X, b, f
Length: 9 amino acids
Theoretical weight: 1.06 KDa
TCR beta chain Chains: E, F, J, N, S, W, a, e
Molecule details ›
Chains: E, F, J, N, S, W, a, e
Length: 111 amino acids
Theoretical weight: 12.04 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
TRAV9-4 Chains: C, D, I, M, R, V, Z, d
Molecule details ›
Chains: C, D, I, M, R, V, Z, d
Length: 109 amino acids
Theoretical weight: 12.07 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5R1C2 (Residues: 22-113; Coverage: 99%)
Gene name: TRAV9-4
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
H-2 class I histocompatibility antigen, L-D alpha chain Chains: A, B, H, L, P, U, Y, c
Molecule details ›
Chains: A, B, H, L, P, U, Y, c
Length: 175 amino acids
Theoretical weight: 20.54 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01897 (Residues: 25-199; Coverage: 52%)
Gene name: H2-L
Structure domains: Murine Class I Major Histocompatibility Complex, H2-DB, subunit A, domain 1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: C222
Unit cell:
a: 158.472Å b: 160.458Å c: 357.159Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.25 0.275
Expression system: Escherichia coli