PDBe 3dw8

X-ray diffraction
2.85Å resolution

Structure of a Protein Phosphatase 2A Holoenzyme with B55 subunit


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform Chains: A, D
Molecule details ›
Chains: A, D
Length: 582 amino acids
Theoretical weight: 64.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P30153 (Residues: 9-589; Coverage: 99%)
Gene name: PPP2R1A
Sequence domains: HEAT repeat
Structure domains: Leucine-rich Repeat Variant
Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform Chains: B, E
Molecule details ›
Chains: B, E
Length: 447 amino acids
Theoretical weight: 51.75 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
  • Canonical: P63151 (Residues: 1-447; Coverage: 100%)
Gene name: PPP2R2A
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform Chains: C, F
Molecule details ›
Chains: C, F
Length: 309 amino acids
Theoretical weight: 35.64 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
  • Canonical: P67775 (Residues: 1-309; Coverage: 100%)
Gene name: PPP2CA
Sequence domains: Calcineurin-like phosphoesterase
Structure domains: Purple Acid Phosphatase; chain A, domain 2
microcystin LR Chains: G, H
Molecule details ›
Chains: G, H
Length: 7 amino acids
Theoretical weight: 1.01 KDa
Source organism: Cyanobacteria
Expression system: Not provided

Ligands and Environments

1 bound ligand:

3 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 247.34Å b: 121.39Å c: 172.48Å
α: 90° β: 132.6° γ: 90°
R R work R free
0.231 0.228 0.285
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda
  • Not provided