PDBe 3dol

X-ray diffraction
2.5Å resolution

Crystal structure of L100I mutant HIV-1 reverse transcriptase in complex with GW695634.

Released:

Function and Biology Details

Reactions catalysed:
Endonucleolytic cleavage to 5'-phosphomonoester. 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). 
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Pol protein Chain: B
Molecule details ›
Chain: B
Length: 440 amino acids
Theoretical weight: 51.4 KDa
Source organism: HIV-1 M:B_HXB2R
Expression system: Escherichia coli
UniProt:
  • Canonical: A7YKL0 (Residues: 161-600; Coverage: 44%)
Gene name: pol
Sequence domains:
Structure domains:
Pol protein Chain: A
Molecule details ›
Chain: A
Length: 560 amino acids
Theoretical weight: 64.59 KDa
Source organism: HIV-1 M:B_HXB2R
Expression system: Escherichia coli
UniProt:
  • Canonical: A7YKL0 (Residues: 161-720; Coverage: 56%)
Gene name: pol
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6, null
Spacegroup: P212121
Unit cell:
a: 139.01Å b: 111.53Å c: 73.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.288
Expression system: Escherichia coli