PDBe 3dmj

X-ray diffraction
2.6Å resolution

CRYSTAL STRUCTURE OF HIV-1 V106A and Y181C MUTANT REVERSE TRANSCRIPTASE IN COMPLEX WITH GW564511.

Released:

Function and Biology Details

Reactions catalysed:
Endonucleolytic cleavage to 5'-phosphomonoester.
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Pol Chain: B
Molecule details ›
Chain: B
Length: 440 amino acids
Theoretical weight: 51.31 KDa
Source organism: HIV-1 M:B_HXB2R
Expression system: Escherichia coli
UniProt:
  • Canonical: Q74596 (Residues: 168-607; Coverage: 43%)
Gene name: pol
Sequence domains:
Structure domains:
Pol protein Chain: A
Molecule details ›
Chain: A
Length: 560 amino acids
Theoretical weight: 64.51 KDa
Source organism: HIV-1 M:B_HXB2R
Expression system: Escherichia coli
UniProt:
  • Canonical: A7YKL0 (Residues: 161-720; Coverage: 56%)
Gene name: pol
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P212121
Unit cell:
a: 137.19Å b: 108.99Å c: 71.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.197 0.284
Expression system: Escherichia coli