PDBe 3d9t

X-ray diffraction
1.5Å resolution

CIAP1-BIR3 in complex with N-terminal peptide from Caspase-9 (ATPFQE)

Released:

Function and Biology Details

Reactions catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Strict requirement for an Asp residue at position P1 and with a marked preference for His at position P2. It has a preferred cleavage sequence of Leu-Gly-His-Asp-|-Xaa
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Baculoviral IAP repeat-containing protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 97 amino acids
Theoretical weight: 11.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13490 (Residues: 260-352; Coverage: 15%)
Gene names: API1, BIRC2, MIHB, RNF48
Sequence domains: Inhibitor of Apoptosis domain
Structure domains: Inhibitor Of Apoptosis Protein (2mihbC-IAP-1); Chain A
Caspase-9 Chains: C, D
Molecule details ›
Chains: C, D
Length: 6 amino acids
Theoretical weight: 692 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P55211 (Residues: 316-321; Coverage: 1%)
Gene names: CASP9, MCH6

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 30.268Å b: 68.427Å c: 122.358Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.183 0.205
Expression systems:
  • Escherichia coli
  • Not provided