PDBe 3d85

X-ray diffraction
1.9Å resolution

Crystal structure of IL-23 in complex with neutralizing FAB

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Interleukin-12 subunit beta Chain: D
Molecule details ›
Chain: D
Length: 306 amino acids
Theoretical weight: 34.75 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P29460 (Residues: 23-328; Coverage: 100%)
Gene names: IL12B, NKSF2
Sequence domains: Cytokine interleukin-12p40 C-terminus
Structure domains: Immunoglobulins
Interleukin-23 subunit alpha Chain: C
Molecule details ›
Chain: C
Length: 178 amino acids
Theoretical weight: 19.67 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9NPF7 (Residues: 20-189; Coverage: 100%)
Gene names: IL23A, SGRF, UNQ2498/PRO5798
Sequence domains: Interleukin 23 subunit alpha
Immunoglobulin heavy constant gamma 1 Chain: B
Molecule details ›
Chain: B
Length: 223 amino acids
Theoretical weight: 23.84 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01857 (Residues: 1-108; Coverage: 33%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
FAB of antibody 7G10, light chain Chain: A
Molecule details ›
Chain: A
Length: 214 amino acids
Theoretical weight: 23.45 KDa
Source organism: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 75.386Å b: 62.091Å c: 107.261Å
α: 90° β: 97.44° γ: 90°
R-values:
R R work R free
0.172 0.171 0.214
Expression system: Trichoplusia ni