PDBe 3crx

X-ray diffraction
2.5Å resolution

CRE RECOMBINASE/DNA COMPLEX INTERMEDIATE I

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 12-mer (preferred)
Entry contents:
1 distinct polypeptide molecule
4 distinct DNA molecules
Macromolecules (5 distinct):
Recombinase cre Chains: A, B
Molecule details ›
Chains: A, B
Length: 343 amino acids
Theoretical weight: 38.57 KDa
Source organism: Escherichia virus P1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06956 (Residues: 1-343; Coverage: 100%)
Gene name: cre
Sequence domains: Phage integrase family
Structure domains:
DNA 35-MER Chain: C
Molecule details ›
Chain: C
Length: 35 nucleotides
Theoretical weight: 10.76 KDa
Source organism: Escherichia virus P1
Expression system: Not provided
DNA 35-MER Chain: D
Molecule details ›
Chain: D
Length: 35 nucleotides
Theoretical weight: 10.72 KDa
Source organism: Escherichia virus P1
Expression system: Not provided
DNA 35-MER Chain: E
Molecule details ›
Chain: E
Length: 35 nucleotides
Theoretical weight: 10.76 KDa
Source organism: Escherichia virus P1
Expression system: Not provided
DNA 35-MER Chain: F
Molecule details ›
Chain: F
Length: 35 nucleotides
Theoretical weight: 10.8 KDa
Source organism: Escherichia virus P1
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2221
Unit cell:
a: 106.3Å b: 122.7Å c: 179.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.268
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided