› 3cla

X-ray diffraction
1.75Å resolution

REFINED CRYSTAL STRUCTURE OF TYPE III CHLORAMPHENICOL ACETYLTRANSFERASE AT 1.75 ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb3cla/pdb
PDB entry 3cla coloured by chain, front view.
PDB entry 3cla coloured by chain, side view.
PDB entry 3cla coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Refined crystal structure of type III chloramphenicol acetyltransferase at 1.75 A resolution.
Leslie AG
J Mol Biol 213 167-86 (1990)
PMID: 2187098

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + chloramphenicol = CoA + chloramphenicol 3-acetate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-132547 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chloramphenicol acetyltransferase 3 Chain: A
Molecule details ›
Chain: A
Length: 213 amino acids
Theoretical weight: 25.02 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P00484 (Residues: 1-213; Coverage: 100%)
Gene name: cat3
Sequence domains: Chloramphenicol acetyltransferase
Structure domains: Chloramphenicol acetyltransferase-like domain

Ligands and Environments

2 bound ligands:
Ligand CO 2 x CO
Ligand CLM 1 x CLM
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: R32
Unit cell:
a: 107.6Å b: 107.6Å c: 123.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.157 0.157 not available
Expression system: Not provided

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Citations

13 review citations

Molecular basis of bacterial resistance to chloramphenicol and florfenicol.
Schwarz et al. (2004)
12 more

19 mentions without citation

A graph-theory algorithm for rapid protein side-chain prediction.
Canutescu et al. (2003)
18 more