PDBe 3cl3

X-ray diffraction
3.2Å resolution

Crystal Structure of a vFLIP-IKKgamma complex: Insights into viral activation of the IKK signalosome

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
FLICE inhibitory protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 183 amino acids
Theoretical weight: 20.67 KDa
Source organism: Human gammaherpesvirus 8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q76RF1 (Residues: 1-178; Coverage: 95%)
Gene name: ORF71
Sequence domains: Death effector domain
Structure domains: Death Domain, Fas
NF-kappa-B essential modulator Chains: D, E
Molecule details ›
Chains: D, E
Length: 130 amino acids
Theoretical weight: 14.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y6K9 (Residues: 150-272; Coverage: 29%)
Gene names: FIP3, IKBKG, NEMO

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P3221
Unit cell:
a: 145.67Å b: 145.67Å c: 101.54Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.261 0.261 0.287
Expression system: Escherichia coli