PDBe 3c5w

X-ray diffraction
2.8Å resolution

Complex between PP2A-specific methylesterase PME-1 and PP2A core enzyme

Released:

Function and Biology Details

Reactions catalysed:
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
[Phosphatase 2A protein]-leucine methyl ester + H(2)O = [phosphatase 2A protein]-leucine + methanol
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform Chain: A
Molecule details ›
Chain: A
Length: 232 amino acids
Theoretical weight: 25.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P30153 (Residues: 9-589; Coverage: 39%)
Gene name: PPP2R1A
Sequence domains: HEAT repeat
Structure domains: Leucine-rich Repeat Variant
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform Chain: C
Molecule details ›
Chain: C
Length: 310 amino acids
Theoretical weight: 35.69 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P67775 (Residues: 1-309; Coverage: 100%)
Gene name: PPP2CA
Sequence domains: Calcineurin-like phosphoesterase
Structure domains: Purple Acid Phosphatase; chain A, domain 2
Protein phosphatase methylesterase 1 Chain: P
Molecule details ›
Chain: P
Length: 310 amino acids
Theoretical weight: 34.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y570 (Residues: 39-386; Coverage: 79%)
  • Best match: Q9Y570-3 (Residues: 5-55, 56-84)
Gene names: PME1, PP2593, PPME1, PRO0750
Sequence domains: Alpha/beta hydrolase family
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 129.292Å b: 54.839Å c: 125.146Å
α: 90° β: 110.87° γ: 90°
R-values:
R R work R free
0.198 0.195 0.263
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda