PDBe 3bzi

X-ray diffraction
2.1Å resolution

Molecular and structural basis of polo-like kinase 1 substrate recognition: Implications in centrosomal localization

Released:

Function and Biology Details

Reactions catalysed:
ATP + a protein = ADP + a phosphoprotein
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein kinase PLK1 Chain: A
Molecule details ›
Chain: A
Length: 239 amino acids
Theoretical weight: 27.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P53350 (Residues: 365-603; Coverage: 40%)
Gene names: PLK, PLK1
Sequence domains: POLO box duplicated region
Structure domains: POLO box domain
M-phase inducer phosphatase 3 Chain: E
Molecule details ›
Chain: E
Length: 9 amino acids
Theoretical weight: 997 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P30307 (Residues: 126-134; Coverage: 2%)
Gene name: CDC25C

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 38.726Å b: 67.481Å c: 87.888Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.174 0.246
Expression systems:
  • Escherichia coli
  • Not provided