PDBe 3buo

X-ray diffraction
2.6Å resolution

Crystal structure of c-Cbl-TKB domain complexed with its binding motif in EGF receptor'


Function and Biology Details

Reactions catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Epidermal growth factor receptor Chains: A, C
Molecule details ›
Chains: A, C
Length: 13 amino acids
Theoretical weight: 1.55 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: NEW P00533 (Residues: 1063-1075; Coverage: 1%)
Gene names: EGFR, ERBB, ERBB1, HER1
E3 ubiquitin-protein ligase CBL Chains: B, D
Molecule details ›
Chains: B, D
Length: 329 amino acids
Theoretical weight: 38.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: NEW P22681 (Residues: 23-351; Coverage: 36%)
Gene names: CBL, CBL2, RNF55
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 63.862Å b: 110.173Å c: 55.821Å
α: 90° β: 89.94° γ: 90°
R R work R free
0.231 0.231 0.278
Expression systems:
  • Not provided
  • Escherichia coli