PDBe 3brf

X-ray diffraction
2.47Å resolution

CSL (Lag-1) bound to DNA with Lin-12 RAM peptide, C2221

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Protein lin-12 Chain: D
Molecule details ›
Chain: D
Length: 14 amino acids
Theoretical weight: 1.65 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: P14585 (Residues: 938-950; Coverage: 1%)
Gene names: R107.8, lin-12
Lin-12 and glp-1 phenotype protein 1, isoform a Chain: A
Molecule details ›
Chain: A
Length: 478 amino acids
Theoretical weight: 53.85 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
Structure domains:
DNA (5'-D(*DAP*DAP*DTP*DCP*DTP*DTP*DTP*DCP*DCP*DCP*DAP*DCP*DAP*DGP*DT)-3') Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.5 KDa
DNA (5'-D(*DTP*DTP*DAP*DCP*DTP*DGP*DTP*DGP*DGP*DGP*DAP*DAP*DAP*DGP*DA)-3') Chain: B
Molecule details ›
Chain: B
Length: 15 nucleotides
Theoretical weight: 4.67 KDa

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: C2221
Unit cell:
a: 62.944Å b: 95.972Å c: 223.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.23 0.277
Expression system: Escherichia coli