PDBe 3bgv

X-ray diffraction
2.3Å resolution

Crystal structure of mRNA cap guanine-N7 methyltransferase in complex with SAH

Released:
Source organism: Homo sapiens
Entry authors: Lunin VV, Wu H, Zeng H, Antoshenko T, MacKenzie F, Weigelt J, Arrowsmith CH, Edwards AM, Bochkarev A, Min J, Plotnikov AN, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(7)G(5')pppR-RNA
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
mRNA cap guanine-N7 methyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 313 amino acids
Theoretical weight: 36.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43148 (Residues: 165-476; Coverage: 66%)
Gene names: KIAA0398, RNMT
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAH 4 x SAH
No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 77.787Å b: 99.672Å c: 167.782Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.221 0.294
Expression system: Escherichia coli