PDBe 3b2u

X-ray diffraction
2.58Å resolution

Crystal structure of isolated domain III of the extracellular region of the epidermal growth factor receptor in complex with the Fab fragment of IMC-11F8

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
IGH@ protein Chains: C, F, H, J, N, Q, T, W
Molecule details ›
Chains: C, F, H, J, N, Q, T, W
Length: 223 amino acids
Theoretical weight: 23.6 KDa
Source organism: Homo sapiens
Expression system: Mus musculus
UniProt:
  • Canonical: Q6GMX6 (Residues: 20-50, 51-116, 117-237; Coverage: 49%)
Gene name: IGH@
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
IMC-11F8 Fab Light chain Chains: D, G, K, L, O, R, U, X
Molecule details ›
Chains: D, G, K, L, O, R, U, X
Length: 213 amino acids
Theoretical weight: 23.05 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Structure domains: Immunoglobulins
Epidermal growth factor receptor Chains: A, B, E, I, M, P, S, V
Molecule details ›
Chains: A, B, E, I, M, P, S, V
Length: 214 amino acids
Theoretical weight: 23.79 KDa
Source organism: Homo sapiens
Expression system: Mus musculus
UniProt:
  • Canonical: P00533 (Residues: 335-538; Coverage: 17%)
  • Best match: P00533-2 (Residues: 335-404)
Gene names: EGFR, ERBB, ERBB1, HER1
Sequence domains: Receptor L domain
Structure domains: 24 nucleotide stem-loop, u2 snrnp hairpin iv. U2 a'. Chain A, domain 1

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P21
Unit cell:
a: 154.373Å b: 139.122Å c: 175.325Å
α: 90° β: 90.02° γ: 90°
R-values:
R R work R free
0.23 0.227 0.291
Expression systems:
  • Mus musculus
  • Spodoptera frugiperda