PDBe 3b1v

X-ray diffraction
1.85Å resolution

Crystal structure of an S. thermophilus NFeoB E67A mutant bound to mGMPPNP

Released:
Primary publication:
A suite of Switch I and Switch II mutant structures from the G-protein domain of FeoB.
Acta Crystallogr. D Biol. Crystallogr. 67 973-80 (2011)
PMID: 22101824

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferrous iron transport protein B Chain: A
Molecule details ›
Chain: A
Length: 272 amino acids
Theoretical weight: 30.15 KDa
Source organism: Streptococcus thermophilus LMG 18311
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5M586 (Residues: 1-270; Coverage: 38%)
Gene names: feoB, stu0608
Sequence domains: Ferrous iron transport protein B
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: C2221
Unit cell:
a: 49.2Å b: 82.7Å c: 150.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.223
Expression system: Escherichia coli