PDBe 3avr

X-ray diffraction
1.8Å resolution

Catalytic fragment of UTX/KDM6A bound with histone H3K27me3 peptide, N-oxyalylglycine, and Ni(II)

Released:
Source organism: Homo sapiens
Primary publication:
Structural basis for histone H3 Lys 27 demethylation by UTX/KDM6A.
Genes Dev. 25 2266-77 (2011)
PMID: 22002947

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Lysine-specific demethylase 6A Chain: A
Molecule details ›
Chain: A
Length: 531 amino acids
Theoretical weight: 60.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW O15550 (Residues: 880-1401; Coverage: 37%)
Gene names: KDM6A, UTX
Sequence domains: JmjC domain, hydroxylase
Structure domains:
Histone H3.1 Chain: B
Molecule details ›
Chain: B
Length: 22 amino acids
Theoretical weight: 2.31 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: NEW P68431 (Residues: 18-39; Coverage: 16%)
Gene names: H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments


1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 78.905Å b: 83.081Å c: 95.057Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.198
Expression systems:
  • Escherichia coli
  • Not provided