PDBe 3agn

X-ray diffraction
0.96Å resolution

Crystal Structure of Ustilago sphaerogena Ribonuclease U2 Complexed with adenosine 3'-monophosphate

Released:

Function and Biology Details

Reaction catalysed:
Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in A-P or G-P with 2',3'-cyclic phosphate intermediates.
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease U2 Chain: A
Molecule details ›
Chain: A
Length: 114 amino acids
Theoretical weight: 12.39 KDa
Source organism: Ustilago sphaerogena
UniProt:
  • Canonical: P00654 (Residues: 1-114; Coverage: 100%)
Gene name: RNU2
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P21
Unit cell:
a: 37.88Å b: 39.789Å c: 35.884Å
α: 90° β: 114.99° γ: 90°
R-values:
R R work R free
0.118 0.117 0.127