PDBe 3a5d

X-ray diffraction
4.8Å resolution

Inter-subunit interaction and quaternary rearrangement defined by the central stalk of prokaryotic V1-ATPase

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
V-type ATP synthase subunit D Chains: G, O
Molecule details ›
Chains: G, O
Length: 223 amino acids
Theoretical weight: 24.72 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: O87880 (Residues: 1-223; Coverage: 100%)
Gene names: TTHA1271, atpD, vatD
Sequence domains: ATP synthase subunit D
V-type ATP synthase subunit F Chains: H, P
Molecule details ›
Chains: H, P
Length: 104 amino acids
Theoretical weight: 11.29 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P74903 (Residues: 1-104; Coverage: 100%)
Gene names: TTHA1274, atpF, vatF
Sequence domains: ATP synthase (F/14-kDa) subunit
V-type ATP synthase beta chain Chains: D, E, F, L, M, N
Molecule details ›
Chains: D, E, F, L, M, N
Length: 478 amino acids
Theoretical weight: 53.22 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q56404 (Residues: 1-478; Coverage: 100%)
Gene names: TTHA1272, atpB
Sequence domains:
V-type ATP synthase alpha chain Chains: A, B, C, I, J, K
Molecule details ›
Chains: A, B, C, I, J, K
Length: 578 amino acids
Theoretical weight: 63.7 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q56403 (Residues: 1-578; Coverage: 100%)
Gene names: TTHA1273, atpA
Sequence domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P321
Unit cell:
a: 380.701Å b: 380.701Å c: 147.971Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.441 0.441 0.454