PDBe 3a4s

X-ray diffraction
2.7Å resolution

The crystal structure of the SLD2:Ubc9 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NFATC2-interacting protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 79 amino acids
Theoretical weight: 8.68 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O09130 (Residues: 339-412; Coverage: 18%)
Gene names: Nfatc2ip, Nip45
Sequence domains: Ubiquitin-2 like Rad60 SUMO-like
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
SUMO-conjugating enzyme UBC9 Chains: A, B
Molecule details ›
Chains: A, B
Length: 163 amino acids
Theoretical weight: 18.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63279 (Residues: 1-158; Coverage: 100%)
Gene names: UBC9, UBCE9, UBE2I
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P1
Unit cell:
a: 29.25Å b: 49.42Å c: 90.295Å
α: 103.2° β: 92.1° γ: 101.13°
R-values:
R R work R free
0.226 0.222 0.269
Expression system: Escherichia coli BL21(DE3)