PDBe 2zus

X-ray diffraction
2.11Å resolution

Crystal structure of Galacto-N-biose/Lacto-N-biose I phosphorylase

Released:

Function and Biology Details

Reaction catalysed:
Beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine + phosphate = alpha-D-galactopyranose 1-phosphate + N-acetyl-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1,3-beta-galactosyl-N-acetylhexosamine phosphorylase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 759 amino acids
Theoretical weight: 85.48 KDa
Source organism: Bifidobacterium longum
Expression system: Escherichia coli
UniProt:
  • Canonical: E8MF13 (Residues: 1-751; Coverage: 100%)
Gene names: BLLJ_1623, lnbp, lnpA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P1
Unit cell:
a: 67.764Å b: 111.483Å c: 118.793Å
α: 105.13° β: 90.22° γ: 107.78°
R-values:
R R work R free
0.173 0.17 0.225
Expression system: Escherichia coli