PDBe 2zth

X-ray diffraction
2.6Å resolution

Crystal structure of holo form of rat catechol-o-methyltransferase

Source organism: Rattus norvegicus
Primary publication:
Crystal structures of the apo and holo form of rat catechol-O-methyltransferase.
J. Struct. Biol. 165 133-9 (2009)
PMID: 19111934

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Catechol O-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 24.92 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
  • Canonical: P22734 (Residues: 44-264; Coverage: 84%)
  • Best match: P22734-2 (Residues: 1-221)
Gene name: Comt
Sequence domains:
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32B2
Spacegroup: P212121
Unit cell:
a: 32.903Å b: 61.089Å c: 105.369Å
α: 90° β: 90° γ: 90°
R R work R free
0.196 0.187 0.281
Expression system: Escherichia coli