› 2zcn

X-ray diffraction
1.9Å resolution

Crystal structure of IcaR, a repressor of the TetR family

Released:
DOI: 10.2210/pdb2zcn/pdb
PDB entry 2zcn coloured by chain, front view.
PDB entry 2zcn coloured by chain, side view.
PDB entry 2zcn coloured by chain, top view.
Source organism: Staphylococcus epidermidis RP62A
Primary publication:
Crystal structure of IcaR, a repressor of the TetR family implicated in biofilm formation in Staphylococcus epidermidis.
Jeng WY, Ko TP, Liu CI, Guo RT, Liu CL, Shr HL, Wang AH
Nucleic Acids Res 36 1567-77 (2008)
PMID: 18208836

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177224 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Biofilm operon icaADBC HTH-type negative transcriptional regulator IcaR Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 192 amino acids
Theoretical weight: 23.16 KDa
Source organism: Staphylococcus epidermidis RP62A
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5HKQ1 (Residues: 1-185; Coverage: 100%)
Gene names: SERP2292, icaR
Sequence domains:
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P21
Unit cell:
a: 39.11Å b: 86.14Å c: 113.85Å
α: 90° β: 99.15° γ: 90°
R-values:
R R work R free
0.172 0.172 0.211
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Current concepts in biofilm formation of Staphylococcus epidermidis.
Fey et al. (2010)
7 more

4 mentions without citation

The TetR family of regulators.
Cuthbertson et al. (2013)
3 more