PDBe 2z98

X-ray diffraction
1.4Å resolution

The crystal structure of AzoR (azoreductase) from Escherichia coli: Oxidized AzoR in tetragonal crystals (The resolution has improved from 1.8 (1v4b) to 1.4 angstrom)

Released:

Function and Biology Details

Reaction catalysed:
N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP(+) = 4-(dimethylamino)azobenzene + 2 NADPH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FMN-dependent NADH-azoreductase Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 21.55 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW P41407 (Residues: 2-201; Coverage: 100%)
Gene names: JW1409, acpD, azoR, b1412
Sequence domains: Flavodoxin-like fold
Structure domains: Rossmann fold

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: P42212
Unit cell:
a: 92.204Å b: 92.204Å c: 51.741Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.22
Expression system: Escherichia coli