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Structure of an IgNAR-AMA1 complex

X-ray diffraction
2.45Å resolution
PDB entry 2z8w coloured by chain, front view.
PDB entry 2z8w coloured by chain, side view.
PDB entry 2z8w coloured by chain, top view.
Source organisms:
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Apical membrane antigen 1 and Ig-like domain-containing protein
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Multimeric state: hetero dimer
Accessible surface area: 21587.37 Å2
Buried surface area: 2102.27 Å2
Dissociation area: 1,051.13 Å2
Dissociation energy (ΔGdiss): 3.01 kcal/mol
Dissociation entropy (TΔSdiss): 12.05 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-180513
Assembly 2
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Assembly Name: Apical membrane antigen 1 and Ig-like domain-containing protein
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Multimeric state: hetero dimer
Accessible surface area: 21209.41 Å2
Buried surface area: 2061.88 Å2
Dissociation area: 1,030.94 Å2
Dissociation energy (ΔGdiss): 2.57 kcal/mol
Dissociation entropy (TΔSdiss): 12.05 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-180513

Macromolecules

Apical membrane antigen 1
Chains: A, B
Length: 335 amino acids
Theoretical weight: 38.36 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7KQK5 (Residues: 104-438; Coverage: 56%)
Gene name: PF3D7_1133400
Pfam: Apical membrane antigen 1
InterPro: Apical membrane antigen 1
CATH: Hepatocyte Growth Factor
Apical membrane antigen 1 in PDB entry 2z8w, assembly 1, front view.
Apical membrane antigen 1 in PDB entry 2z8w, assembly 1, side view.
Apical membrane antigen 1 in PDB entry 2z8w, assembly 1, top view.
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Ig-like domain-containing protein
Chains: C, D
Length: 116 amino acids
Theoretical weight: 12.86 KDa
Source organism: Orectolobus maculatus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6X1E6 (Residues: 1-113; Coverage: 100%)
Pfam: Immunoglobulin V-set domain
InterPro:
CATH: Immunoglobulins
Ig-like domain-containing protein in PDB entry 2z8w, assembly 1, front view.
Ig-like domain-containing protein in PDB entry 2z8w, assembly 1, side view.
Ig-like domain-containing protein in PDB entry 2z8w, assembly 1, top view.
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