PDBe 2z5h

X-ray diffraction
2.89Å resolution

Crystal structure of the head-to-tail junction of tropomyosin complexed with a fragment of TnT

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Cellular component:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
homo dimer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Troponin T, fast skeletal muscle isoforms Chain: T
Molecule details ›
Chain: T
Length: 55 amino acids
Theoretical weight: 6.67 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P12620 (Residues: 58-112; Coverage: 21%)
Gene name: TNNT3
Tropomyosin alpha-1 chain; General control protein GCN4 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 52 amino acids
Theoretical weight: 6.06 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P58772 (Residues: 234-234, 254-284; Coverage: 11%)
  • Canonical: P03069 (Residues: 260-278; Coverage: 7%)
  • nullnull
Gene names: AAS3, ARG9, GCN4, TPM1, TPMA, YEL009C
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
General control protein GCN4; Tropomyosin alpha-1 chain Chain: I
Molecule details ›
Chain: I
Length: 40 amino acids
Theoretical weight: 4.54 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P58772 (Residues: 1-24; Coverage: 9%)
  • Canonical: P03069 (Residues: 267-278; Coverage: 4%)
  • nullnull
Gene names: AAS3, ARG9, GCN4, TPM1, TPMA, YEL009C

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: I212121
Unit cell:
a: 86.809Å b: 158.308Å c: 163.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.237 0.247
Expression system: Escherichia coli