2ypw Experiments and Validation

Electron Microscopy

Source organism: Escherichia coli
Resolution: 12.4Å

Sample information

Author description:
Atomic model for the N-terminus of TraO fitted in the full-length structure of the bacterial pKM101 type IV secretion system core complex
Source organism: Escherichia coli
Expression system: Escherichia coli BL21

Validation information

Experimental information

Specimen preparation
pH: 8
Buffer: 50 MM TRIS-HCL, 200 MM NACL, 10 MM LDAO
Vitrification
Details: VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 60, TEMPERATURE- 92, INSTRUMENT- NONE, METHOD- BLOT 3 SECONDS BEFORE PLUNGING,
Refinement statistics
Structure solution method: Electron Microscopy
Resolution: 12.4 Å