2yjt › Citations

Potential regulatory interactions of Escherichia coli RraA protein with DEAD-box helicases.

Pietras Z, Hardwick SW, Swiezewski S, Luisi BF
J Biol Chem 288 31919-29 (2013)
Cited: 11 times
EuropePMC logo PMID: 24045937

Abstract

Members of the DEAD-box family of RNA helicases contribute to virtually every aspect of RNA metabolism, in organisms from all domains of life. Many of these helicases are constituents of multicomponent assemblies, and their interactions with partner proteins within the complexes underpin their activities and biological function. In Escherichia coli the DEAD-box helicase RhlB is a component of the multienzyme RNA degradosome assembly, and its interaction with the core ribonuclease RNase E boosts the ATP-dependent activity of the helicase. Earlier studies have identified the regulator of ribonuclease activity A (RraA) as a potential interaction partner of both RNase E and RhlB. We present structural and biochemical evidence showing how RraA can bind to, and modulate the activity of RhlB and another E. coli DEAD-box enzyme, SrmB. Crystallographic structures are presented of RraA in complex with a portion of the natively unstructured C-terminal tail of RhlB at 2.8-Å resolution, and in complex with the C-terminal RecA-like domain of SrmB at 2.9 Å. The models suggest two distinct mechanisms by which RraA might modulate the activity of these and potentially other helicases.

Articles - 2yjt mentioned but not cited (1)

  1. Potential regulatory interactions of Escherichia coli RraA protein with DEAD-box helicases. Pietras Z, Hardwick SW, Swiezewski S, Luisi BF. J Biol Chem 288 31919-31929 (2013)


Reviews citing this publication (4)

  1. Bacterial versatility requires DEAD-box RNA helicases. Redder P, Hausmann S, Khemici V, Yasrebi H, Linder P. FEMS Microbiol Rev 39 392-412 (2015)
  2. Analytical ultracentrifugation: A versatile tool for the characterisation of macromolecular complexes in solution. Patel TR, Winzor DJ, Scott DJ. Methods 95 55-61 (2016)
  3. Trans-acting regulators of ribonuclease activity. Lee J, Lee M, Lee K. J Microbiol 59 341-359 (2021)
  4. Trans-acting regulators of ribonuclease activity. Lee J, Lee M, Lee K. J Microbiol (2021)

Articles citing this publication (6)

  1. The C-terminal region of the RNA helicase CshA is required for the interaction with the degradosome and turnover of bulk RNA in the opportunistic pathogen Staphylococcus aureus. Giraud C, Hausmann S, Lemeille S, Prados J, Redder P, Linder P. RNA Biol 12 658-674 (2015)
  2. SrmB Rescues Trapped Ribosome Assembly Intermediates. Rabuck-Gibbons JN, Popova AM, Greene EM, Cervantes CF, Lyumkis D, Williamson JR. J Mol Biol 432 978-990 (2020)
  3. RraAS1 inhibits the ribonucleolytic activity of RNase ES by interacting with its catalytic domain in Streptomyces coelicolor. Seo S, Kim D, Song W, Heo J, Joo M, Lim Y, Yeom JH, Lee K. J Microbiol 55 37-43 (2017)
  4. The DEAD-box RNA helicase RhlE2 is a global regulator of Pseudomonas aeruginosa lifestyle and pathogenesis. Hausmann S, Gonzalez D, Geiser J, Valentini M. Nucleic Acids Res 49 6925-6940 (2021)
  5. Digested disorder, Quarterly intrinsic disorder digest (October-November-December, 2013). DeForte S, Reddy KD, Uversky VN. Intrinsically Disord Proteins 3 e984569 (2015)
  6. Both Enolase and the DEAD-Box RNA Helicase CrhB Can Form Complexes with RNase E in Anabaena sp. Strain PCC 7120. Yan H, Qin X, Wang L, Chen W. Appl Environ Microbiol 86 e00425-20 (2020)


Quick links