› 2yca

X-ray diffraction
1.8Å resolution

Mixed-function P450 MycG in complex with mycinamicin III in P21212 space group

Released:
DOI: 10.2210/pdb2yca/pdb
PDB entry 2yca coloured by chain, front view.
PDB entry 2yca coloured by chain, side view.
PDB entry 2yca coloured by chain, top view.
Source organism: Micromonospora griseorubida
Primary publication:
Substrate recognition by the multifunctional cytochrome P450 MycG in mycinamicin hydroxylation and epoxidation reactions.
Li S, Tietz DR, Rutaganira FU, Kells PM, Anzai Y, Kato F, Pochapsky TC, Sherman DH, Podust LM
J Biol Chem 287 37880-90 (2012)
PMID: 22952225

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176804 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mycinamicin IV hydroxylase/epoxidase Chain: A
Molecule details ›
Chain: A
Length: 417 amino acids
Theoretical weight: 46.56 KDa
Source organism: Micromonospora griseorubida
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q59523 (Residues: 1-397; Coverage: 100%)
Gene name: mycG
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

4 bound ligands:
Ligand HEM 1 x HEM
Ligand ZM3 1 x ZM3
Ligand GOL 3 x GOL
Ligand SIN 1 x SIN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21212
Unit cell:
a: 56.747Å b: 162.33Å c: 49.789Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.152 0.21
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Expansion of chemical space for natural products by uncommon P450 reactions.
Zhang et al. (2017)
4 more

5 mentions without citation

New reactions and products resulting from alternative interactions between the P450 enzyme and redox partners.
Zhang et al. (2014)
4 more