› 2yba

X-ray diffraction
2.55Å resolution

Crystal structure of Nurf55 in complex with histone H3

Released:
DOI: 10.2210/pdb2yba/pdb
PDB entry 2yba coloured by chain, front view.
PDB entry 2yba coloured by chain, side view.
PDB entry 2yba coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Histone methylation by PRC2 is inhibited by active chromatin marks.
Schmitges FW, Prusty AB, Faty M, Stützer A, Lingaraju GM, Aiwazian J, Sack R, Hess D, Li L, Zhou S, Bunker RD, Wirth U, Bouwmeester T, Bauer A, Ly-Hartig N, Zhao K, Chan H, Gu J, Gut H, Fischle W, Müller J, Thomä NH
Mol Cell 42 330-41 (2011)
PMID: 21549310

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135452 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chromatin assembly factor 1 p55 subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 422 amino acids
Theoretical weight: 47.76 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q24572 (Residues: 1-418; Coverage: 97%)
Gene names: CG4236, Caf1, Caf1-55
Sequence domains:
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Histone H3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 19 amino acids
Theoretical weight: 2.08 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided
UniProt:
  • Canonical: P02299 (Residues: 2-20; Coverage: 14%)
Gene names: CG31613, CG33803, CG33806, CG33809, CG33812, CG33815, CG33818, CG33821, CG33824, CG33827, CG33830, CG33833, CG33836, CG33839, CG33842, CG33845, CG33848, CG33851, CG33854, CG33857, CG33860, CG33863, CG33866, His3, His3:CG31613, His3:CG33803, His3:CG33806, His3:CG33809, His3:CG33812, His3:CG33815, His3:CG33818, His3:CG33821, His3:CG33824, His3:CG33827, His3:CG33830, His3:CG33833, His3:CG33836, His3:CG33839, His3:CG33842, His3:CG33845, His3:CG33848, His3:CG33851, His3:CG33854, His3:CG33857, His3:CG33860, His3:CG33863, His3:CG33866

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 55.98Å b: 88.16Å c: 204.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.222
Expression systems:
  • Trichoplusia ni
  • Not provided

Quick links

Citations

150 review citations

Occupying chromatin: Polycomb mechanisms for getting to genomic targets, stopping transcriptional traffic, and staying put.
Simon et al. (2013)
149 more

16 mentions without citation

Perceiving the epigenetic landscape through histone readers.
Musselman et al. (2012)
15 more