PDBe 2ya5

X-ray diffraction
2Å resolution

Crystal structure of Streptococcus pneumoniae NanA (TIGR4) in complex with sialic acid

Released:

Function and Biology Details

Reaction catalysed:
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates. 
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sialidase A Chains: A, B
Molecule details ›
Chains: A, B
Length: 493 amino acids
Theoretical weight: 55.35 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P62575 (Residues: 318-792; Coverage: 48%)
Gene name: nanA
Sequence domains: BNR repeat-like domain
Structure domains: Neuraminidase

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: C2
Unit cell:
a: 165.272Å b: 49.251Å c: 125.255Å
α: 90° β: 104.84° γ: 90°
R-values:
R R work R free
0.21 0.208 0.271
Expression system: Escherichia coli BL21