PDBe 2y8y

X-ray diffraction
1.44Å resolution

Structure B of CRISPR endoribonuclease Cse3 bound to 19 nt RNA

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
CRISPR-associated endoribonuclease Cse3 Chain: A
Molecule details ›
Chain: A
Length: 215 amino acids
Theoretical weight: 24.05 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q53WG9 (Residues: 1-211; Coverage: 100%)
Gene names: TTHB192, cas6, cse3
Sequence domains: CRISPR associated protein
Structure domains:
5'-R(*UP*CP*CP*CP*CP*AP*CP*GP*CP*GP*UP*GP*UP*GP *GP*GP*DGP*AP*U)-3' Chain: B
Molecule details ›
Chain: B
Length: 19 nucleotides
Theoretical weight: 6.07 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 44.28Å b: 71.21Å c: 76.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.184 0.205
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided