› 2y5j

X-ray diffraction
2.33Å resolution

Crystal structure of Burkholderia cenocepacia dihydropteroate synthase.

Released:
DOI: 10.2210/pdb2y5j/pdb
PDB entry 2y5j coloured by chain, front view.
PDB entry 2y5j coloured by chain, side view.
PDB entry 2y5j coloured by chain, top view.
Source organism: Burkholderia cenocepacia
Primary publication:
Crystal structures of Burkholderia cenocepacia dihydropteroate synthase in the apo-form and complexed with the product 7,8-dihydropteroate.
Morgan RE, Batot GO, Dement JM, Rao VA, Eadsforth TC, Hunter WN
BMC Struct Biol 11 21 (2011)
PMID: 21554707

Function and Biology Details

Reaction catalysed: 
6-hydroxymethyl-7,8-dihydropterin diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109990 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydropteroate synthase Chain: A
Molecule details ›
Chain: A
Length: 294 amino acids
Theoretical weight: 31 KDa
Source organism: Burkholderia cenocepacia
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B4E5F5 (Residues: 1-292; Coverage: 100%)
Gene names: BCAL1268, dhpS, folP
Sequence domains: Pterin binding enzyme
Structure domains: Dihydropteroate synthase-like

Ligands and Environments

1 bound ligand:
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2221
Unit cell:
a: 73.952Å b: 89.433Å c: 87.596Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.207 0.274
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Utility of the Biosynthetic Folate Pathway for Targets in Antimicrobial Discovery.
Bourne CR. (2014)
2 more

1 mention without citation

Crystal structures of Burkholderia cenocepacia dihydropteroate synthase in the apo-form and complexed with the product 7,8-dihydropteroate.
Morgan et al. (2011)