› 2y58

X-ray diffraction
3.25Å resolution

Fragment growing induces conformational changes in acetylcholine- binding protein: A structural and thermodynamic analysis - (Compound 6)

Released:
DOI: 10.2210/pdb2y58/pdb
PDB entry 2y58 coloured by chain, front view.
PDB entry 2y58 coloured by chain, side view.
PDB entry 2y58 coloured by chain, top view.
Source organism: Aplysia californica
Primary publication:
Fragment growing induces conformational changes in acetylcholine-binding protein: a structural and thermodynamic analysis.
Edink E, Rucktooa P, Retra K, Akdemir A, Nahar T, Zuiderveld O, van Elk R, Janssen E, van Nierop P, van Muijlwijk-Koezen J, Smit AB, Sixma TK, Leurs R, de Esch IJ
J Am Chem Soc 133 5363-71 (2011)
PMID: 21322593

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neurotransmitter-gated ion-channel ligand-binding domain-containing protein Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 217 amino acids
Theoretical weight: 24.69 KDa
Source organism: Aplysia californica
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8WSF8 (Residues: 20-236; Coverage: 100%)
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains: Neurotransmitter-gated ion-channel ligand-binding domain

Ligands and Environments

3 bound ligands:
Ligand V38 5 x V38
Ligand CL 6 x CL
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: I23
Unit cell:
a: 213.33Å b: 213.33Å c: 213.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.169 0.198
Expression system: Spodoptera frugiperda

Quick links

Citations

8 review citations

Structural insights into Cys-loop receptor function and ligand recognition.
Nys et al. (2013)
7 more

1 mention without citation

Size matters in activation/inhibition of ligand-gated ion channels.
Du et al. (2012)