› 2x9w

X-ray diffraction
1.92Å resolution

STRUCTURE OF THE PILUS BACKBONE (RRGB) FROM STREPTOCOCCUS PNEUMONIAE

Released:
DOI: 10.2210/pdb2x9w/pdb
PDB entry 2x9w coloured by chain, front view.
PDB entry 2x9w coloured by chain, side view.
PDB entry 2x9w coloured by chain, top view.
Source organism: Streptococcus pneumoniae TIGR4
Primary publication:
Supramolecular organization of the repetitive backbone unit of the Streptococcus pneumoniae pilus.
Spraggon G, Koesema E, Scarselli M, Malito E, Biagini M, Norais N, Emolo C, Barocchi MA, Giusti F, Hilleringmann M, Rappuoli R, Lesley S, Covacci A, Masignani V, Ferlenghi I
PLoS One 5 e10919 (2010)
PMID: 20559564

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell wall surface anchor family protein Chain: A
Molecule details ›
Chain: A
Length: 444 amino acids
Theoretical weight: 48.21 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2UNM7 (Residues: 184-627; Coverage: 70%)
Gene name: SP_0463
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 3 x GOL
1 modified residue:
Ligand MSE 1 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 67.36Å b: 74.063Å c: 104.461Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.228
Expression system: Escherichia coli

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Citations

8 review citations

Architects at the bacterial surface - sortases and the assembly of pili with isopeptide bonds.
Hendrickx et al. (2011)
7 more

8 mentions without citation

Structural biology of Gram-positive bacterial adhesins.
Vengadesan et al. (2011)
7 more