› 2x9d

X-ray diffraction
2.34Å resolution

Tet repressor (class D) in complex with iso-7-chlortetracycline

Released:
DOI: 10.2210/pdb2x9d/pdb
PDB entry 2x9d coloured by chain, front view.
PDB entry 2x9d coloured by chain, side view.
PDB entry 2x9d coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Recognition of drug degradation products by target proteins: isotetracycline binding to Tet repressor.
Volkers G, Petruschka L, Hinrichs W
J Med Chem 54 5108-15 (2011)
PMID: 21699184

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142298 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tetracycline repressor protein class D Chain: A
Molecule details ›
Chain: A
Length: 207 amino acids
Theoretical weight: 23.29 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACT4 (Residues: 2-208; Coverage: 95%)
Gene name: tetR
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ITC 1 x ITC
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: I4122
Unit cell:
a: 68.253Å b: 68.253Å c: 179.271Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.234 0.28
Expression system: Escherichia coli

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Citations

1 review citation

Transcription factor-based biosensors enlightened by the analyte.
Fernandez-López et al. (2015)
2 other articles