› 2x78

X-ray diffraction
2Å resolution

Human foamy virus integrase - catalytic core.

Released:
DOI: 10.2210/pdb2x78/pdb
PDB entry 2x78 coloured by chain, front view.
PDB entry 2x78 coloured by chain, side view.
PDB entry 2x78 coloured by chain, top view.
Source organism: Human spumaretrovirus
Primary publication:
Structural studies of the catalytic core of the primate foamy virus (PFV-1) integrase.
Réty S, Reaeábková L, Dubanchet B, Silhán J, Legrand P, Lewit-Bentley A
Acta Crystallogr Sect F Struct Biol Cryst Commun 66 881-6 (2010)
PMID: 20693659

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146935 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Integrase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 200 amino acids
Theoretical weight: 22.52 KDa
Source organism: Human spumaretrovirus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14350 (Residues: 861-1060; Coverage: 18%)
Gene name: pol
Sequence domains: Integrase core domain
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: C2221
Unit cell:
a: 51.8Å b: 98.04Å c: 240.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.212 0.272
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Porcine endogenous retrovirus-A/C: biochemical properties of its integrase and susceptibility to raltegravir.
Demange et al. (2015)

2 mentions without citation

Structural biology of retroviral DNA integration.
Li et al. (2011)
1 more