› 2x5k

X-ray diffraction
2.37Å resolution

Structure of an active site mutant of the D-Erythrose-4-Phosphate Dehydrogenase from E. coli

Released:
DOI: 10.2210/pdb2x5k/pdb
PDB entry 2x5k coloured by chain, front view.
PDB entry 2x5k coloured by chain, side view.
PDB entry 2x5k coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Moniot S, Didierjean C, Boschi-Muller S, Branlant G, Corbier C

Function and Biology Details

Reaction catalysed: 
D-erythrose 4-phosphate + NAD(+) + H(2)O = 4-phosphoerythronate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141865 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-erythrose-4-phosphate dehydrogenase Chains: O, P, Q, R
Molecule details ›
Chains: O, P, Q, R
Length: 339 amino acids
Theoretical weight: 37.3 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0A9B6 (Residues: 1-339; Coverage: 100%)
Gene names: JW2894, b2927, epd, gapB
Sequence domains:
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand GOL 6 x GOL
Ligand EDO 5 x EDO
Ligand PG4 1 x PG4
Ligand CL 3 x CL
Ligand PGE 4 x PGE
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P41212
Unit cell:
a: 134.81Å b: 134.81Å c: 246.11Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.164 0.207
Expression system: Escherichia coli K-12

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