PDBe 2x3t

X-ray diffraction
2.75Å resolution

Glutaraldehyde-crosslinked wheat germ agglutinin isolectin 1 crystal soaked with a synthetic glycopeptide

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GLYCOPEPTIDE Chains: E, M
Molecule details ›
Chains: E, M
Length: 9 amino acids
Theoretical weight: 886 Da
Source organism: synthetic construct
Expression system: Not provided
Agglutinin isolectin 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 171 amino acids
Theoretical weight: 17.12 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: P10968 (Residues: 27-197; Coverage: 92%)
Sequence domains: Chitin recognition protein
Structure domains: Wheat Germ Agglutinin (Isolectin 2); domain 1

Ligands and Environments

3 bound ligands:

3 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 44.64Å b: 93.21Å c: 92.5Å
α: 90° β: 98.32° γ: 90°
R-values:
R R work R free
0.232 0.228 0.285
Expression system: Not provided