PDBe 2x2l

X-ray diffraction
2Å resolution

Crystal Structure of phosphorylated RET tyrosine kinase domain with inhibitor

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble RET kinase fragment Chain: A
Molecule details ›
Chain: A
Length: 314 amino acids
Theoretical weight: 35.79 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P07949 (Residues: 703-1013; Coverage: 29%)
Gene names: CDHF12, CDHR16, PTC, RET, RET51
Sequence domains: Protein tyrosine kinase
Structure domains:

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2
Unit cell:
a: 71.999Å b: 70.685Å c: 71.573Å
α: 90° β: 110.35° γ: 90°
R-values:
R R work R free
0.214 0.211 0.269
Expression system: Spodoptera frugiperda