› 2x17

X-ray diffraction
3.1Å resolution

The X-ray structure of Ferritin from Pyrococcus furiosus loaded with Ag(I)

Released:
DOI: 10.2210/pdb2x17/pdb
PDB entry 2x17 coloured by chain, front view.
PDB entry 2x17 coloured by chain, side view.
PDB entry 2x17 coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Silver ion incorporation and nanoparticle formation inside the cavity of Pyrococcus furiosus ferritin: structural and size-distribution analyses.
Kasyutich O, Ilari A, Fiorillo A, Tatchev D, Hoell A, Ceci P
J Am Chem Soc 132 3621-7 (2010)
PMID: 20170158

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186446 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferritin-like diiron domain-containing protein Chains: 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, G, H, I, J, K, L, M, N, O, P, Q, R, Y, Z
Molecule details ›
Chains: 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, G, H, I, J, K, L, M, N, O, P, Q, R, Y, Z
Length: 173 amino acids
Theoretical weight: 20.2 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8U2T8 (Residues: 1-173; Coverage: 99%)
Gene name: PF0742
Sequence domains: Ferritin-like domain
Structure domains: Ferritin

Ligands and Environments

1 bound ligand:
Ligand AG 33 x AG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P41
Unit cell:
a: 157.681Å b: 157.681Å c: 246.437Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.261 0.26 0.283
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Biomimetic and Bioinspired Synthesis of Nanomaterials/Nanostructures.
Zan et al. (2016)
11 more

7 mentions without citation

Harnessing Nanoparticles for Immunomodulation and Vaccines.
Gomes et al. (2017)
6 more