› 2x0u

X-ray diffraction
1.6Å resolution

STRUCTURE OF THE P53 CORE DOMAIN MUTANT Y220C BOUND TO A 2-amino substituted benzothiazole scaffold

Released:
DOI: 10.2210/pdb2x0u/pdb
PDB entry 2x0u coloured by chain, front view.
PDB entry 2x0u coloured by chain, side view.
PDB entry 2x0u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Toward the rational design of p53-stabilizing drugs: probing the surface of the oncogenic Y220C mutant.
Basse N, Kaar JL, Settanni G, Joerger AC, Rutherford TJ, Fersht AR
Chem Biol 17 46-56 (2010)
PMID: 20142040

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138105 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellular tumor antigen p53 Chains: A, B
Molecule details ›
Chains: A, B
Length: 219 amino acids
Theoretical weight: 24.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04637 (Residues: 94-312; Coverage: 56%)
Gene names: P53, TP53
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand X0U 1 x X0U
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 65.06Å b: 71.14Å c: 105.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.165 0.193
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Drugging the p53 pathway: understanding the route to clinical efficacy.
Khoo et al. (2014)
17 more

3 mentions without citation

Screening out anti-inflammatory or anti-viral targets in Xuanfei Baidu Tang through a new technique of reverse finding target.
Wang et al. (2021)
2 more